منابع مشابه
Ultrafiltration and Displacement Chromatography
Although apparently the first effective parathyroid extract was prepared by Moussu in 1898, some time elapsed before Hanson (1926) and Collip (1925) clearly demonstrated that an active extract of consistent potency could be prepared by using hot dilute hydrochloric acid. Since that time there have been a number of attempts to purify such extracts further. Some further purification was achieved ...
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Ion-exchange displacement chromatography has been adapted to centrifugal partition chromatography. The use of an ionic liquid, benzalkonium chloride, as a strong anion-exchanger has proven to be efficient for the preparative separation of phenolic acid regioisomers. Multigram quantities of a mixture of three hydroxycinnamic acid isomers were separated using iodide as a displacer. The displaceme...
متن کاملDisplacement Chromatography on Synthetic Ion - exchange Resins
In Part 1 of this series of papers (Partridge & Westall, 1949) it was shown that, whilst the baseexchange resin Zeo Karb 215 may be used with success for the separation of the neutral and acidic amino-acids, certain difficulties arise when the separation of basic substances is attempted. These difficulties were traced to the phenolic hydroxyl groups of the resin. Resins of the sulphonated pheno...
متن کاملAmpholyte-displacement chromatography for the separation of serum proteins.
The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were t...
متن کاملPreparative isolation of amino acids by carrier displacement chromatography on ion exchange resins.
Chromatographic separations of amino acids on ion exchange resins may be performed on a preparative scale either by elution (24) or by displacement (5-12). Some of the exceptional resolving power demonstrated in analytical separations by elution techniques (13, 14) disappears when larger columns with relatively higher loads are used (2). Displacement chromatography allows large quantities of so...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2003
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/41.6.281